I just tried a Highpoint RocketU 1022C in two PCIe 1.0 motherboards:
one detected that card in Device Manager, but I would not install a working device driver
the other never detected that card at all, despite being a more modern ASUS workstation motherboard
I’m thinking that USB 3.0 AICs were NOT seriously contemplated until PCIe 2.0 increased the raw clock rate from 2.5G to 5G per x1 lane:
5G / 10 bits per byte = 500 MB/second, using the former “legacy frame”:
1 start bit + 8 data bits + 1 stop bit.
5G is the standard clock rate for good ol’ USB 3.0 ports:
https://en.wikipedia.org/wiki/USB_3.0
“Transfer speed – USB 3.0 adds a new transfer type called SuperSpeed or SS, 5 Gbit/s“